产品详情
This antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins.
免疫原详细信息
Purified recombinant human FEN-1 protein fragments expressed in E.coli.
相关试剂
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
背景
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions
