发布时间：2025-02-26
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文章标题
超声引导下星状神经节阻滞
治疗老年失眠症的效果
作者：
顾宇，岳馨，韩翃，吴贤，罗文，徐涛
作者单位：
长春中医药大学临床医学院药理学教研室
发表期刊：
中国老年学杂志
DOI：
10.3969/j. issn.1005-9202.2024.03.022
关键词：
失眠症; 超声引导; 星状神经节阻滞; 睡眠质量
备注：
乙酰胆碱、5-羟色胺；
引用优品生物试剂盒
科研乙酰胆碱酯酶(AchE)试剂盒；
科研5-羟色胺(5-HT)检测试剂盒 ；
文献推荐
IF5.7
文章标题
"Stigmasterol Activates the mTOR Signaling Pathway by Inhibiting
ORP5 Ubiquitination to Promote Milk Synthesis in Bovine Mammary
Epithelial Cells"
作者：
Mingyang Sun, Yu Cao, Ji Cheng, Dianwen Xu, Feng Li, Jiaxin Wang, Yusong Ge, Yuhao Liu,Xiaoyu Long, Wenjin Guo, Juxiong Liu,and Shoupeng Fu
作者单位：
国家诊断和治疗严重人畜共患传染病重点实验室，教育部人畜共患病研究重点实验室，吉林省大学严重人畜共患传染病诊疗国家重点实验室；
DOI：
org/10.1021/acs.jafc.4c0324
发表期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
关键词：
BMECs, stigmasterol, ORP5, mTOR signaling pathway, MARCH4, ubiquitination
文章摘要
Stigmasterol (ST), a phytosterol found in food, has various biological activities. However, the effect of ST on milk
synthesis in dairy cows remains unclear. Therefore, bovine primary mammary epithelial cells (BMECs) were isolated, cultured, andtreated with ST to determine the effect of ST on milk synthesis. The study revealed that 10 μM ST significantly increased milksynthesis in BMECs by activating the mammalian target of rapamycin (mTOR) signaling pathway. Further investigation revealedthat this activation depends on the regulatory role of oxysterol binding protein 5 (ORP5). ST induces the translocation of ORP5from the cytoplasm to the lysosome, interacts with the mTOR, recruits mTOR to target the lysosomal surface, and promotes theactivation of the mTOR signaling pathway. Moreover, ST was found to increase ORP5 protein levels by inhibiting its degradation viathe ubiquitin-proteasome pathway. Specifically, the E3 ubiquitin ligase membrane-associated cycle-CH-type finger 4 (MARCH4)promotes the ubiquitination and subsequent degradation of ORP5. ST mitigates the interaction between MARCH4 and ORP5,thereby enhancing the structural stability of ORP5 and reducing its ubiquitination. In summary, ST stabilizes ORP5 by inhibiting theinteraction between MARCH4 and ORP5, thereby activating mTOR signaling pathway and enhancing milk synthesis.
引用优品生物试剂盒
科研原料-MARCH4 Polyclonal Antibody；
