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作者：冯艳霞,岳燕军,张宏燕,宁建峰

降脂蛋白；

趋化素；

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作者：易知非，罗雪峰，何佳奇，吾路汗·马汗，段赟，谢增如

高敏 C 反应蛋白( hs-CRP）；

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作者：孙 晗,孙雅楠,谌治安

NF-kB P65；

cleaved Caspase-3；

Bcl-2；

Bax 抗体；

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关键词：electrochemiluminescence,porphyrins, ferrocene, regulation, B4C, AFP    

Development of nanoluminophores with high efficiency, good stability, and excellent electronic transfer ability is one
of the key challenges in electrochemiluminescence (ECL). Here, a porphyrin-based nanomaterial named Fc-TCPP was synthesizedby self-assembly of 1,1′-ferrocenedicarboxylic acid (Fc(COOH)2) and tetrakis-(4-carboxyphenyl) porphyrin (TCPP) under infraredheating light. By tuning the ratio of the precursors, the morphology of Fc-TCPP can be regulated from primitive nanospheres tothorn-ball flowers decorated with branches and particles, and the possible formation mechanism was proposed. Nano-boron carbide(B4C) with carbon defect sites further boosts the ECL performance of Fc-TCPP, and thereby, an ECL biosensor for AFP wasconstructed innovatively. This study provides a simple and effective strategy for the preparation of ECL nanoluminophores takingthe advantages of ferrocene and TCPP, opening a new horizon to design porphyrin-based nanomaterials with various structures andexpanding their application in biosensing and disease diagnosis.    

抗人糖类抗原199（CA199）抗体；

carcinoembryonic antigen(CEA)；

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Pyroptosis is a type of regulated cell death executed by gasdermin family members. However, how gasdermin-mediated pyroptosisis negatively regulated remains unclear. Here, we demonstrate that mannose, a hexose, inhibits GSDME-mediated pyroptosis byactivating AMP-activated protein kinase (AMPK). Mechanistically, mannose metabolism in the hexosamine biosynthetic pathwayincreases levels of the metabolite N-acetylglucosamine-6-phosphate (GlcNAc-6P), which binds AMPK to facilitate AMPKphosphorylation by LKB1. Activated AMPK then phosphorylates GSDME at Thr6, which leads to blockade of caspase-3-inducedGSDME cleavage, thereby repressing pyroptosis. The regulatory role of AMPK-mediated GSDME phosphorylation was furtherconfirmed in AMPK knockout and GSDMET6E or GSDMET6A knock-in mice. In mouse primary cancer models, mannose administrationsuppressed pyroptosis in small intestine and kidney to alleviate cisplatin- or oxaliplatin-induced tissue toxicity without impairingantitumor effects. The protective effect of mannose was also verified in a small group of patients with gastrointestinal cancer whoreceived normal chemotherapy. Our study reveals a novel mechanism whereby mannose antagonizes GSDME-mediated pyroptosisthrough GlcNAc-6P-mediated activation of AMPK, and suggests the utility of mannose supplementation in alleviatingchemotherapy-induced side effects in clinic applications.    

甘露糖（D-Mannose）含量测定试剂盒；