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BID (phospho Ser78) Polyclonal Antibody

货号: YP-Ab-00147
促销价:

产品介绍

反应种属
Human;Mouse
应用范围
IHC;IF;ELISA
抗体类型
多克隆抗体
基因名称(Gene Name)
BID
蛋白名称
BH3-interacting domain death agonist
分子量(DA)
免疫原
The antiserum was produced against synthesized peptide derived from human BID around the phosphorylation site of Ser78. AA range:44-93
特异性
Phospho-BID (S78) Polyclonal Antibody detects endogenous levels of BID protein only when phosphorylated at S78.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源
Polyclonal, Rabbit,IgG
稀释比例
WB 1:500-2000 ,IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
浓度
1 mg/ml
储存
-20°C/1 year
其他名称
BID; BH3-interacting domain death agonist; p22 BID; BID
背景
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],
功能
domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.,subcellular location:A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.,subcellular location:Associated with the mitochondrial membrane.,subcellular location:Translocates to mitochondria as an integral membrane protein.,subcellular location:When uncleaved

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注:我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存,效价高的抗体可至少保存1周,至少重复利用3次。

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