反应种属
Human;Mouse;Rat;Monkey
免疫原
Purified recombinant human PKM2 protein fragments expressed in E.coli.
特异性
This antibody detects endogenous levels of PKM2 and does not cross-react with related proteins.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
wb 1:1000 icc 1:400. IF 1:50-200
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
其他名称
CTHBP;Cytosolic thyroid hormone binding protein;Cytosolic thyroid hormone-binding protein;KPYM_HUMAN;MGC3932;OIP 3;OIP-3;OIP3;OPA interacting protein 3;Opa-interacting protein 3;p58;PK muscle type;PK, muscle type;PK2;PK3;PKM;PKM2;pykm;Pyruvate kinase 2/3;Pyruvate kinase 3;Pyruvate kinase isozymes M1/M2;Pyruvate kinase muscle;Pyruvate kinase muscle isozyme;pyruvate kinase PKM;Pyruvate kinase, muscle 2;TCB;THBP1;Thyroid hormone binding protein 1;Thyroid hormone binding protein cytosolic;Thyroid hormone-binding protein 1;Tumor M2 PK;Tumor M2-PK.
背景
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011],
功能
catalytic activity:ATP + pyruvate = ADP + phosphoenolpyruvate.,cofactor:Divalent metal cations.,cofactor:Magnesium.,cofactor:Potassium.,enzyme regulation:Isoform M2 is allosterically activated by D-fructose 1,6-biphosphate (FBP). Inhibited by oxalate and 3,3',5-triiodo-L-thyronine (T3).,function:Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP.,miscellaneous:There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells.,online information:Pyruvate kinase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Be
