免疫原
Purified recombinant human SIRT1 protein fragments expressed in E.coli.
特异性
This antibody detects endogenous levels of SIRT1 and does not cross-react with related proteins.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
其他名称
75SirT1;BA57G10.4;BA57G10.4;hSIR2;hSIRT1;HST2, S. cerevisiae, homolog of;NAD dependent deacetylase SIRT1;NAD dependent deacetylase sirtuin 1;NAD dependent protein deacetylase sirtuin 1;OTTHUMP00000198111;OTTHUMP00000198112;Regulatory protein SIR2 homolog 1;SIR1_HUMAN;SIR2 like 1;SIR2 like protein 1;SIR2, S.cerevisiae, homolog-like 1;SIR2-like protein 1;SIR2ALPHA;SIR2ALPHA;SIR2alpha protein;SIR2alpha protein;SIR2L1;SIRT 1;Sirt1;SIRT1 Sir2 like proteins (siruitins) type 1;SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);SirtT1 75 kDa fragment;sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);Sirtuin 1; sirtuin;Sirtuin type 1;Sirtuin type 1.
背景
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],
功能
catalytic activity:NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.,cofactor:Binds 1 zinc ion per subunit.,enzyme regulation:Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). RPS19BP1/AROS acts as a positive regulator of deacetylation activity.,function:NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1
