免疫原
Purified recombinant human BRAP protein fragments expressed in E.coli.
特异性
This antibody detects endogenous levels of BRAP and does not cross-react with related proteins.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
其他名称
3010002G07Rik;BRAP2;BRCA1 associated protein;EC 6.3.2.;Galectin 2 binding protein;IMP; Impedes mitogenic signal propagation;Renal carcinoma antigen NY REN 63; RING finger protein 52;RNF52;zgc:92894.
背景
The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. [provided by RefSeq, Jul 2008],
功能
function:Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 UBP-type zinc finger.,subunit:Interacts with the nuclear localization signal of BRCA1 and with the N-terminal of KSR1. The C-terminal portion of BCRA1 interacts with DDB1.,tissue specificity:Expressed in breast epithelial cell lines.,
