免疫原
Purified recombinant human B Raf protein fragments expressed in E.coli.
特异性
This antibody detects endogenous levels of B Raf and does not cross-react with related proteins.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
其他名称
FLJ95109;94 kDa B raf protein;B raf 1;B Raf proto oncogene serine threonine protein kinase;B-Raf proto-oncogene serine/threonine-protein kinase (p94);BRAF 1;BRAF;BRAF_HUMAN;BRAF1;cRmil;MGC126806;MGC138284;Murine sarcoma viral (v-raf) oncogene homolog B1;Murine sarcoma viral v raf oncogene homolog B1;NS7;oncogene BRAF1;p94;Proto-oncogene B-Raf;Proto-oncogene c-Rmil;RAFB 1;RAFB1;RMIL;Serine/threonine-protein kinase B-raf;v raf murine sarcoma viral oncogene homolog B;v raf murine sarcoma viral oncogene homolog B1;v-Raf murine sarcoma viral oncogene homolog B1
背景
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008],
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2 zinc ions per subunit.,disease:Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC synd
