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作者：张宝文，李颖，寇现娟    

lba1 Polyclonal Antibody；

TREM2 Polyclonal Antibody；

作者：黄安敏

C 反应蛋白（CRP）试剂盒；

作者：徐小玉，卢冬冬  

IL－6、TNF－α

Immune checkpoint blockade (ICB) therapy using anti-programmed cell death 1 (PD1) and cytotoxic T lymphocyte antigen 4 (CTLA4), either individual or in combination, has emerged as a pioneer in clinical immunotherapy for various human cancers. However, immune-related adverse effects (irAEs) remain the sig-nificant hindrances to their clinical development. In particular, immune-related myocarditis has emerged as a fatal adverse event with the highest fatality rate. For this purpose, we report herein the first example of uncoupling efficacy and myocarditis toxicity in checkpoint blockade cancer therapy. This efficient, yet highly safe access to ICB therapy was achieved by a simple nanoplatform that masked anti-PD1/CTLA4 antibodies to albumin via a ROS-cleavable linker. An in-depth investigation of the experimental autoimmune myocarditis (EAM) mouse model revealed the avoiding exacerbation of myocarditis of our nanomedicine. Notably, the on- demand release of antibodies at the tumor sites enables the combined checkpoint antibody activity. The pre-sent study uncouples the ICB efficacy and toxicity, and highlights the irresistible charm of TME-responsive nanoantibodies in clinical cancer immunotherapy, offering solutions to challenges in the advancement of TME-responsive drug delivery toward clinical application.    

小鼠免疫球蛋白 G2b（IgG2b）；

大鼠免疫球蛋白 G2a（IgG2a）；

Prostate-specific antigen (PSA) is a key marker for a
prostate cancer diagnosis. The low sensitivity of traditional lateralflow immunoassay (LFIA) methods makes them unsuitable forpoint-of-care testing. Herein, we designed a nanozyme by in situgrowth of Prussian blue (PB) within the pores of dendriticmesoporous silica (DMSN). The PB was forcibly dispersed into thepores of DMSN, leading to an increase in exposed active sites.Consequently, the atom utilization is enhanced, resulting insuperior peroxidase (POD)-like activity compared to that ofcubic PB. Antibody-modified DMSN@PB nanozymes serve asimmunological probes in an enzymatic-enhanced colorimetric andphotothermal dual-signal LFIA for PSA detection. After systematicoptimization, the LFIA based on DMSN@PB successfully achievesa 4-fold amplification of the colorimetric signal within 7 min through catalytic oxidation of the chromogenic substrate by POD-likeactivity. Moreover, DMSN@PB exhibits an excellent photothermal conversion ability under 808 nm laser irradiation. Accordingly,photothermal signals are introduced to improve the anti-interference ability and sensitivity of LFIA, exhibiting a wide linear range(1−40 ng mL−1) and a low PSA detection limit (0.202 ng mL−1), which satisfies the early detection level of prostate cancer. Thisresearch provides a more accurate and reliable visualization analysis methodology for the early diagnosis of prostate cancer.    

Fibronectin  polyclonal antibody
collagen I polyclonal antibody,
 N-cadherin polyclonal antibody
 TGF-β1 polyclonal antibody,
α-SMA polyclonal antibody
 GAPDH polyclonal antibody, and
HRP-sheep anti  mouse IgG (H + L)